Generator

Part:BBa_K3395001

Designed by: YONG JI   Group: iGEM20_RDFZ-China   (2020-10-23)


musTPH1

musTPH1 is the enzyme that can hydroxylate tryptophan to 5-HTP(5-hydroxytryptophan) different from TPH1(BBa_K33950000). This enzyme is present in mice.

Design

musTPH1 is derived from mouse.RDFZ-China also collected a mouse-sourced musTPH1 and plan for the same characterization and testing. It is known to catalyze the same reaction human TPH1 did. Although the full experimentation data haven’t been complete on this biobrick, musTPH1 is proven to have high expression in the bacteria strain E.coli MC1061 with proper activity to produce concentration greater than 1mM under certain conditions. musTPH1 is construct on pET28-b plasmid and bind with upstream LacI sequence. We transformed pET28b_musTPH1 vector intoE coli BL21 for expression.

Biosensor circuit of pET28b_musTPH1

Reference

Park DH,Stone DM, Kim KS, et al. Characterization of recombinant mouse tryptophan hydroxylase expressed in Escherichia coli[J]. Molecular and Cellular Neuroscience, 1994, 5(1): 87-93.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 191
    Illegal XhoI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 249


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